Klára Hilscherová, Ph.D.

Klára Hilscherová, Ph.D.

Specialization:

  • biochemical mechanisms and in vitro effects of xenobiotics including cyanotoxins
  • aquatic ecotoxicology

Contacts:

e-mail:

Education:

  • M.Sc. in Environmental Protection (Faculty of Science, Masaryk University Brno, 1995)
  • Ph.D. in Environmental Chemistry (Faculty of Science, Masaryk University Brno, 2000)
  • ERASMUS stipendium in Research Institute of Toxicology (RITOX, 1995), University of Utrecht, Netherlands
  • stipendium in Aquatic Toxicology Laboratory, Michigan State University, USA (1997-1999, 1997-1998 Fulbright stipendium)

Other activities:

  • TOCOEN Ltd., Brno - research worker (1999-2005)
  • RECETOX (Faculty of Science, Masaryk University Brno) - research worker (since 2004)
  • Institute of Botany, Academy of Sciences of Czech Republic - research assistant (since 2005)
  • Michigan State University, National Food Safety and Toxicology, East Lansing, USA - consultant/post-doctoral (2001-2003)
  • Member of SETAC (Society of Environmental Toxicology and Chemistry)

Selected publications:

  • Mazurova, E., Hilscherova, K., Jalova, V., Kohler, H.R., Triebskorn, R., Giesy, J.P., Blaha, L. (2008): Endocrine effects of contaminated sediments on the freshwater snail Potamopyrgus antipodarum in vivo and in the cell bioassays in vitro. Aquatic Toxicology 89(3): 172-179.
  • Haeba, M.H., Hilscherova, K., Mazurova, E., Blaha, L. (2008): Selected endocrine disrupting compounds (vinclozolin, flutamide, ketoconazole and dicofol): Effects on survival, occurrence of males, growth, molting and reproduction of Daphnia magna. Environmental Science and Pollution Research. 15(3): 222-227.
  • Mazurova, E., Hilscherova, K., Triebskorn, R., Kohler, H.R., Marsalek, B., Blaha, L. (2008): Endocrine regulation of the reproduction in crustaceans - identification of potential targets for toxicants and environmental contaminants. Biologia 63(2): 139-150.
  • Gracia, T., Hilscherova, K., Jones, P.D., Newsted, J.L., Higley, E.B., Zhang, X., Hecker, M., Murphy, M., Yu, R.M.K., Lam, P.K.S., Wu, R.S.S., Giesy J.P. (2007): Modulation of steroidogenic gene expression and hormone production of H295R cells by pharmaceuticals and other environmentally active compounds. Toxicology and Applied Pharmacology 225: 142-153.
  • Haeba, M., Hilscherova K., Mazurova, E., Blaha, L. (2007): Selected endocrine disrupting compounds (vinclozolin, flutamide, ketoconazole and dicofol): Effects on survival, occurrence of males, growth, molting and reproduction of Daphnia magna. Environmental Science and Pollution Research - International. 6(3): 1-6. (ISSN 1614-7499)
  • Zounkova, R., Odraska, P., Dolezalová, L., Hilscherova K., Marsalek, B., Blaha, L. (2007): Ecotoxicity and genotoxicity assessment of cytostatic pharmaceuticals. Environmental Toxicology and Chemistry 26(10): 2208-2214.
  • Adamovsky, O., Kopp, R., Hilscherova, K., Babica, P., Palikova, M., Paskova, V., Navratil, S., Marsalek, B., Blaha, L. (2007): Microcystins kinetics (bioaccumulation, elimination) and biochemical responses in common carp and silver carp exposed to toxic cyanobacterial blooms. Environmental Toxicology and Chemistry 26(12): 2687-2693.
  • Skocovska, B., Hilscherova, K., Babica, P., Adamovsky, O., Bandouchová, H., Horakova, J., Knotkova, Z., Marsalek, B., Paskova, V., Pikula, J. (2007): Effects of cyanobacterial biomass on the Japanese quail. Toxicon 49(6): 793-803.
  • Novak, J., Benisek, M., Pachernik, J., Janosek, J., Sidlová, T., Kiviranta, H., Verta, M., Giesy, J.P., Blaha, L., Hilscherova, K. (2007): Interference of contaminated sediment extracts and environmental pollutants with retinoid signaling. Environmental Toxicology and Chemistry 26(8): 1591-1599.
  • Bittner, M., Hilscherova, K., Giesy, J.P. (2007): Changes of AhR-mediated activity of humic substance after irradiation. Environment International 33(6): 812-816.
  • Hilscherova, K., Dusek L., Kubik, V., Hofman, J., Cupr, P., Klanova, J., Holoubek, I. (2007): Redistribution of organic pollutants in river sediments and alluvial soils related to major floods. Journal of Soils and Sediments 7(3): 167-177.
  • Babica, P., Hilscherova, K., Bartova, K., Blaha, L., Marsalek, B. (2007): Effects of dissolved microcystins on growth of planktonic photoautotrophs. Phycologia 46(2) 137-142.
  • Janosek, J., Bittner, M., Hilscherova, K., Blaha, L., Giesy, J.P., Holoubek, I. (2007): AhR-mediated and antiestrogenic activity of humic substances. Chemosphere 67(6): 1096-1101.
  • Palikova, M., Krejci, R., Hilscherova, K., Babica, P., Navratil, S., Kopp, R., Blaha, L. (2007): Effect of different cyanobacterial biomasses and their fractions with variable microcystin content on embryonal development of carp (Cyprinus carpio L.). Aquatic Toxicology 81(3): 312-318.
  • Palikova, M., Krejci, R., Hilscherova, K., Buryskova, B., Babica, P., Navratil, S., Kopp, R., Blaha, L. (2007): Effects of different oxygen saturation on activity of complex biomass and aqueous crude extract of cyanobacteria during embryonal development in carp (Cyprinus carpio L.). Acta Veterinaria Brno 76(2): 291?299.
  • Gracia, T., Hilscherova, K., Jones, P.D., Newsted, J.L., Zhang, X., Hecker, M., Higley, E.B., Sanderson, T., Yu, R.M.K., Wu, R.S.S., Giesy J.P. (2006): The H295R system for evaluation of endocrine-disrupting effects. Ecotoxicology and Environmental Safety 65(3): 293-305.
  • Janosek J., Hilscherova K., Blaha, L., Holoubek I. (2006): Environmental xenobiotics and nuclear receptors - Interactions, effects and in vitro assessment. Toxicology In Vitro 20(1): 18-37.
  • Blaha, L., Hilscherova, K., Mazurova, E., Hecker, M., Jones, P.D., Newsted, J.L., Bradley, P. W., Gracia, T., Duris, Z., Horka, I., Holoubek, I., Giesy, J.P. (2006): Alteration of steroidogenesis in H295R cells by organic sediment contaminants and relationships to other endocrine disrupting effects. Environment International 32(6): 749-757.
  • Bittner, M., Janosek, J., Hilscherova, K., Giesy, J.P., Holoubek, I., Blaha, L. (2006): Activation of Ah receptor by pure humic acids. Environmental Toxicology 21(4): 338-342.
  • Feldmannova, M., Hilscherova, K., Marsalek, B., Blaha, L. (2006): Effect of N-heterocyclic polyaromatic hydrocarbons (N-PAHs) on survival, reproduction and biochemicals parameters in Daphnia magna. Environmental Toxicology 21(4): 425-431.
  • Buryskova, B., Hilscherova, K., Blaha, L., Marsalek, B., Holoubek, I. (2006): Toxicity and modulations of biomarkers in Xenopus laevis embryos exposed to polycyclic aromatic hydrocarbons and their N-heterocyclic derivatives. Environmental Toxicology 21(1): 590-598.
  • Buryskova, B., Hilscherova, K., Babica, P., Vrskova, D., Marsalek, B., Blaha, L. (2006): Toxicity of complex cyanobacterial samples and their fractions in Xenopus laevis embryos and the role of microcystins. Aquatic Toxicology 80(4): 346-354.
  • Paskova, V., Hilscherova, K., Feldmannova, M., Blaha, L. (2006): Toxic effects and oxidative stress in higher plants exposed to polycyclic aromatic hydrocarbons and their N-heterocyclic derivatives. Environmental Toxicology and Chemistry 25(12): 3238-3245.
  • Sovadinova, I., Blaha, L., Janosek, J., Hilscherova, K., Giesy, J.P., Jones, P.D., Holoubek, I. (2006): Cytotoxicity and Aryl hydrocarbon receptor-mediated activity of N-heterocyclic polycyclic aromatic hydrocarbons - structure-activity relationships. Environmental Toxicology and Chemistry 25(5): 1291-1297.
  • Smid, R., Kubasek, M., Klimes, D., Dusek, L., Jarkovsky, J., Marsalek, B., Hilscherova, K., Blaha, L., Cupr, P., Holoubek, I. (2006): Web portal for management of bioindication methods and ecotoxicological tests in ecological risk assessment. Ecotoxicology 15(8): 623-627.
  • Zhang, X.W., Yu, R.M.K., Jones, P.D., Lam, G.K.W., Newsted, J.L., Gracia, T., Hecker, M., Hilscherova, K., Sanderson, J.T., Wu, R.S.S, Giesy, J.P. (2005): Quantitative RT-PCR methods for evaluating toxicant-induced effects on steroidogenesis using the H295R cell line. Environmental Science and Technology 39(8): 2777-2785.
  • Hilscherova, K., Jones, P.D., Gracia, T., Newsted, J.L., Zhang, X., Sanderson, J.T., Yu, R., Wu, R., Giesy, J.P. (2004): Quantitative assessment of the effects of chemicals on steroidogenic enzymes in the H295R cell line using real-time quantitative PCR. Toxicological Sciences 81(1): 78-89.
  • Hilscherova, K., Blankenship, A.L., Kannan, K., Nie, M., Williams, L.L., Coady, K., Upham, B.L., Trosko, J.E., Bursian, S.J., Giesy, J.P. (2003): Oxidative stress in laboratory-incubated double-crested cormorant eggs collected from the Great Lakes. Archives of Environmental Contamination and Toxicology 45(4): 533-546.
  • Blankenship, A.L., Hilscherova, K., Nie, M., Coady, K.K., Villalobos, S.A., Kannan, K., Powell, D.C., Bursian, S.J., Giesy J.P. (2003): Mechanisms of TCDD-induced abnormalities and embryo lethality in white leghorn chickens. Comparative Biochemistry and Physiology C - Toxicology and Pharmacology 136(1): 47-62.
  • Hilscherova, K., Blankenship, A.L., Nie, M., Coady, K.K., Upham, B.L., Trosko, J.E., Giesy, J.P. (2003): Oxidative stress in liver and brain of the hatchling chicken (Gallus domesticus) following in ovo injection with TCDD. Comparative Biochemistry and Physiology C - Toxicology and Pharmacology 136(1): 29-45.
  • Hilscherova, K., Kannan, K., Nakata, H., Hanari, N., Yamashita, N., Bradley, P.W., McCabe, J.M., Taylor, A.B., Giesy, J.P. (2003): Polychlorinated dibenzo-p-dioxin and dibenzofuran concentration profiles in sediments and flood-plain soils of the Tittabawassee River, Michigan. Environmental Science and Technology 37(3): 468-474.
  • Hilscherova, K., Kannan, K., Holoubek, I., Giesy, J.P. (2002): Characterization of estrogenic activity of riverine sediments from the Czech Republic. Archives of Environmental Contamination and Toxicology 43(2): 175-185.
  • Kannan, K., Hilscherova, K., Imagawa, T., Yamashita, N., Williams, L.L., Giesy, J.P. (2001): Polychlorinated naphthalenes, -biphenyls, -dibenzo-p-dioxins, and -dibenzofurans in double-crested cormorants and herring gulls from Michigan waters of the Great Lakes. Environmental Science and Technology 35(3): 441-447.
  • Hilscherova, K., Kannan, K., Kang, Y.-S., Holoubek, I., Machala, M., Masunaga, S., Nakanishi, J., Giesy J.P. (2001): Characterization of dioxin-like activity of sediments from a Czech river basin. Environmental Toxicology and Chemistry 20(12): 2768-2777.
  • Machala, M., Dusek, L., Hilscherova, K., Kubinova, R., Neca, J., Ulrich, R., Jurajda, P., Gelnar, M., Studnickova, Z., Holoubek, I. (2001): Determination and multivariate statistical analysis of biochemical responses to environmental contaminants in feral freshwater fish (Leuciscus cephalus L.). Environmental Toxicology and Chemistry 20(5): 1141-1148.
  • Machala, M., Hilscherova, K., Kubinova, R., Ulrich, R., Vykusova, B., Kolarova, J., Machova, J., Svobodova, Z. (2000): Biochemical markers of dioxin-like toxicity and oxidative stress in hepatic microsomes of bream (Abramis brama) and perch (Perca fluviatilis) in the Elbe River. Veterinární Medicína 45(7): 195-199.
  • Hilscherova, K., Machala, M., Kannan, K., Blankenship, A.L., Giesy, J.P. (2000): Cell bioassays for detection of aryl hydrocarbon (AhR) and estrogen receptor (ER) mediated activity in environmental samples. Environmental Science and Pollution Research 7(3): 159-171.
  • Holoubek, I., Machala, M., Staffova, K., Helesic, J., Ansorgova, A., Schramm, K.W., Kettrup, A., Giesy, J.P., Kannan, K., Mitera, J. (1998): PCDD/Fs in sediments from Morava river catchment area. Organochlorine Compounds 39: 261-266.


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